[Histonet] Histonet Digest, Vol 148, Issue 20 processing

[Steve McClain]

I don't really like wasting valuable rapid-processing time on fixation, when I cab icontrol that at the bench 
Your schedule is made more difficult because of the length of time in formalin. See if your director can ensure only fixed tissue on processor. If not increase temp to 45C station #1 and change frequently allowing only 1 hour in warm formalin. This will  gain an 45 min for 100% and paraffin (two stages where you are disproportionately short).
Then remove the formalin station #2  replace w 50% alc 15 minutes rinses buffer salts off the seals lengthening life of processor seals.

3 75% alc 30
4 95% alc decrease to 15 min
5 95% alc 45 min
6 100% alc increase to 15 min
7 100% alc decrease to 30 min
8 100 % alc increase to 45
9 xylene 1 decrease to 15
10 xylene 2 same 30
11 zylene 3 increase to 45
12 paraffin 1 decrease to 15
13 paraffin 2 same 30
14 paraffin 3 increase to 90 min
 Should give you just under 9 hours.
Since you are constrained on time and depend on consistent, rapid diffusion, rotate solutions frequently. 

Steve A. McClain, MD

> On Mar 22, 2016, at 13:53, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
> 
> 1. Formalin   1hr
> 2. Formalin   1hr
> 3. 75% alcohol 30 mins
> 4.85% alcohol 30 mins
> 5. 95% alcohol 30 mins
> 6.95% alcohol 30 mins
> 7. 100% alcohol 30 mins
> 8. 100% alcohol 30 mins
> 9 xylene 30 mins
> 10. xylene 30 mins
> 11 xylene 30 mins
> 12. Wax for 30 mins
> 13. Wax 30 mins
> 14. Wax 30 minutes.