[Histonet] congo red - decolor

[Jay Lundgren]

Decolorize Congo Red by soaking in a 1% alcoholic solution of Sodium
Hydroxide.  Heat and stirring might help, be careful not to soak your
sections off the slides, NaOH is a strong base.

               Jay A. Lundgren, M.S., HTL (ASCP)

On Thu, Mar 17, 2016 at 1:13 PM, Nancy Schmitt <Nancy_Schmitt at pa-ucl.com>
wrote:

> Hi Jay-
>
>
>
> It really is Congo Red!
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> Thank you for responding so quickly,
>
>
>
> Nancy
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>
>
> Nancy Schmitt MLT, HT(ASCP)
>
> Pathology Support Services Manager
>
> United Clinical Laboratories
>
> 205 Bluff Street, Suite 1
>
> Dubuque, IA  52001
>
> Ph. 563-690-4142
>
> Check us out at www.uclaccess.com
>
>
>
>
>
>
>
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>
> *From:* Jay Lundgren [mailto:jaylundgren at gmail.com]
> *Sent:* Thursday, March 17, 2016 13:10 PM
> *To:* Nancy Schmitt
> *Cc:* histonet at lists.utsouthwestern.edu
> *Subject:* Re: [Histonet] congo red - decolor
>
>
>
> Are you sure you don't mean Oil Red O stain for fat?  Just checking.
>
>
>
>  Congo Red is for amyloid.
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> Destain Congo Red by soaking in 100% Propylene Glycol, warmth and stirring
> would speed it up.
>
>
>
>                               Jay A. Lundgren, M.S., HTL (ASCP)
>
>
>
> On Thu, Mar 17, 2016 at 12:41 PM, Nancy Schmitt via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> Happy St. Pat's Day!
>
> Any thoughts on how to decolorize Congo Red stains that were done on fat
> pad slides?
> We appreciate any insight you might offer:)
>
> Nancy
> United Clinical Labs
> Dubuque, IA
>
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