[Histonet] Marking tissue with eosin

lisa ryan haemeotoxylin at hotmail.com
Fri Jun 24 14:43:45 CDT 2016


We add 50mls of 1% eosin to our first alcohol after the formalin.

1% EOSIN
FOR PROCESSING BIOPSIES

100mL ACETIC ACID
2g EOSIN
100mL DISTILLED WATER

Stir well!

Lisa Ryan 
Histology 
St James hospital 
Dublin Ireland

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Sent: 24 June 2016 17:00
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Subject: Histonet Digest, Vol 151, Issue 22

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Today's Topics:

   1. Marking Tissues with Eosin (Rebecca E. Ashley)
   2. Re: Marking Tissues with Eosin (Mca Werdler)
   3. Re: Shandon Varistain Gemini Slide Stainer (Teri Johnson)
   4. Re: Marking Tissues with Eosin (Jamal Rowaihi)
   5. Re: Shandon Varistain Gemini Slide Stainer (Paula Keene Pierce)
   6. Re: Marking Tissues with Eosin (Cindy Bird)
   7. Re: Marking Tissues with Eosin (Cynthia Robinson)


----------------------------------------------------------------------

Message: 1
Date: Thu, 23 Jun 2016 17:04:19 +0000
From: "Rebecca E. Ashley" <Pez at uwyo.edu>
To: "Histonet at lists.utsouthwestern.edu"
        <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Marking Tissues with Eosin
Message-ID:
        <BLUPR05MB1874458C42D648382309FB96DB2D0 at BLUPR05MB1874.namprd05.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

I had a biopsy today that was nearly impossible to see on the sponges during embedding or in the block.  I've heard mention of marking these with eosin to make them easier to see.  Has anyone done this?  Or do you use some other type of marking dye for this purpose?
Thanks for your input!
Rebecca

Rebecca Ashley
Histotechnologist
Wyoming State Vet Lab
1174 Snowy Range Rd.
Laramie, WY 82070
Phone: 307-766-9946
Fax: 307-721-2051



------------------------------

Message: 2
Date: Thu, 23 Jun 2016 12:49:37 -0500
From: Mca Werdler <mwerdler at gmail.com>
To: "Rebecca E. Ashley" <Pez at uwyo.edu>
Cc: "Histonet at lists.utsouthwestern.edu"
        <Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID:
        <CAM7pV2mh7bt2T7npM5p=vfhV487=1KG3R2VcfL_DtVqF0dRRYw at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Dear Rebecca,

Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.

Good luck,

Maarten

2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet at lists.utsouthwestern.edu>:

> I had a biopsy today that was nearly impossible to see on the sponges
> during embedding or in the block.  I've heard mention of marking these with
> eosin to make them easier to see.  Has anyone done this?  Or do you use
> some other type of marking dye for this purpose?
> Thanks for your input!
> Rebecca
>
> Rebecca Ashley
> Histotechnologist
> Wyoming State Vet Lab
> 1174 Snowy Range Rd.
> Laramie, WY 82070
> Phone: 307-766-9946
> Fax: 307-721-2051
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 3
Date: Thu, 23 Jun 2016 19:04:44 +0000
From: Teri Johnson <tejohnson at genoptix.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer
Message-ID:
        <d4d1b863c94a4982b41652bb37e199fe at PHUSCB-SP37MB01.genoptix.org>
Content-Type: text/plain; charset=WINDOWS-1252

Hi Thomas,

Are you looking for true life expectancy or what is reported for depreciation? In my experience, most tissue processors never die, but only need to be retired due to lack of available support/parts or because a lab requires newer technology.

Also in my experience, a "better" model comes out soon after I have purchased one. So the life span is probably 20 years after you wished you had a different one. :-)

Best wishes,

Teri

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohnson at genoptix.com
www.genoptix.com

------------------------------

Hi All,
Does anyone know the life expectancy of the Gemini H&E stainer? My boss has asked me this question because we are in the process of requesting for a new one.

Thomas

Thomas Huynh   BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | Thomas.Huynh at harrishealth.org

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.

To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.  This e-mail may also be confidential and/or privileged under Texas law.  The e-mail is for the use of only the individual or entity named above.  If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.








------------------------------

Message: 4
Date: Thu, 23 Jun 2016 22:56:34 +0300
From: Jamal Rowaihi <j.rowaihi at alborglaboratories.com>
To: Mca Werdler <mwerdler at gmail.com>, "Rebecca E. Ashley"
        <Pez at uwyo.edu>
Cc: ???? ???????        <j.rowaihi at alborglaboratories.com>,
        "Histonet at lists.utsouthwestern.edu"
        <Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID: <pktvvaa0mu6cb5ppytq4gb9u.1466711448386 at email.android.com>
Content-Type: text/plain; charset=utf-8

Hi?If you are using buffered Formaline so the Eosin color will not resist until the end of tissue processing.I recommend to add small amount of stock Eosin to the last alcohol in theTissue processor.?

Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone
-------- Original message --------From: Mca Werdler via Histonet <histonet at lists.utsouthwestern.edu> Date: 6/23/16  8:49 PM  (GMT+03:00) To: "Rebecca E. Ashley" <Pez at uwyo.edu> Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Marking Tissues with Eosin
Dear Rebecca,

Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.

Good luck,

Maarten

2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet at lists.utsouthwestern.edu>:

> I had a biopsy today that was nearly impossible to see on the sponges
> during embedding or in the block.? I've heard mention of marking these with
> eosin to make them easier to see.? Has anyone done this?? Or do you use
> some other type of marking dye for this purpose?
> Thanks for your input!
> Rebecca
>
> Rebecca Ashley
> Histotechnologist
> Wyoming State Vet Lab
> 1174 Snowy Range Rd.
> Laramie, WY 82070
> Phone: 307-766-9946
> Fax: 307-721-2051
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Thu, 23 Jun 2016 21:02:49 +0000 (UTC)
From: Paula Keene Pierce <paula at excaliburpathology.com>
To: Teri Johnson <tejohnson at genoptix.com>,      Histonet
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer
Message-ID:
        <994846485.545115.1466715770021.JavaMail.yahoo at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

This is so true!
I had a Fisher Histomatic automated slide stainer that was manufactured in 1985 that I used until the building took a direct lightning strike JUST LAST YEAR!
My old VIP is still going!
?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

      From: Teri Johnson via Histonet <histonet at lists.utsouthwestern.edu>
 To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu>
 Sent: Thursday, June 23, 2016 2:04 PM
 Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer

Hi Thomas,

Are you looking for true life expectancy or what is reported for depreciation? In my experience, most tissue processors never die, but only need to be retired due to lack of available support/parts or because a lab requires newer technology.

Also in my experience, a "better" model comes out soon after I have purchased one. So the life span is probably 20 years after you wished you had a different one. :-)

Best wishes,

Teri

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA? 92008
USA

Phone +1 760 516 5954
tejohnson at genoptix.com
www.genoptix.com

------------------------------

Hi All,
Does anyone know the life expectancy of the Gemini H&E stainer? My boss has asked me this question because we are in the process of requesting for a new one.

Thomas

Thomas Huynh? BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | Thomas.Huynh at harrishealth.org

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.

To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.






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Histonet at lists.utsouthwestern.edu
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------------------------------

Message: 6
Date: Thu, 23 Jun 2016 16:20:45 -0500
From: Cindy Bird <CBird at amli-denton.com>
To: Mca Werdler <mwerdler at gmail.com>
Cc: "Rebecca E. Ashley" <Pez at uwyo.edu>,
        "Histonet at lists.utsouthwestern.edu"
        <Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID: <DEFA943F-8F9A-4A22-80C1-29D4F9ACEBD6 at amli-denton.com>
Content-Type: text/plain; charset="us-ascii"

We place a small drop of concentrate straight on tissue.

Sent from my iPhone

> On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> Dear Rebecca,
>
> Yes this is possible. just don't use a too strong concentration. The eosin
> should give a slight pink color on the tissue after processing and after
> embedding.
>
> Good luck,
>
> Maarten
>
> 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
> histonet at lists.utsouthwestern.edu>:
>
>> I had a biopsy today that was nearly impossible to see on the sponges
>> during embedding or in the block.  I've heard mention of marking these with
>> eosin to make them easier to see.  Has anyone done this?  Or do you use
>> some other type of marking dye for this purpose?
>> Thanks for your input!
>> Rebecca
>>
>> Rebecca Ashley
>> Histotechnologist
>> Wyoming State Vet Lab
>> 1174 Snowy Range Rd.
>> Laramie, WY 82070
>> Phone: 307-766-9946
>> Fax: 307-721-2051
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Fri, 24 Jun 2016 13:19:43 +0000
From: Cynthia Robinson <robinsoc at mercyhealth.com>
To: Cindy Bird <CBird at amli-denton.com>, Mca Werdler
        <mwerdler at gmail.com>
Cc: "Histonet at lists.utsouthwestern.edu"
        <Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID:
        <4EE642D353925D4D96CB95E12427DBAE56DD4CF9 at NODCMSTMBX06.no.trinity-health.org>

Content-Type: text/plain; charset="us-ascii"

We use safranin at the grossing station and it is a dark pink at embedding. Works really well in our hands. Added plus is no fluorescent issues that you can have with eosin.

Cindi

________________________________________
From: Cindy Bird via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Thursday, June 23, 2016 4:20 PM
To: Mca Werdler
Cc: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Marking Tissues with Eosin

We place a small drop of concentrate straight on tissue.

Sent from my iPhone

> On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> Dear Rebecca,
>
> Yes this is possible. just don't use a too strong concentration. The eosin
> should give a slight pink color on the tissue after processing and after
> embedding.
>
> Good luck,
>
> Maarten
>
> 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
> histonet at lists.utsouthwestern.edu>:
>
>> I had a biopsy today that was nearly impossible to see on the sponges
>> during embedding or in the block.  I've heard mention of marking these with
>> eosin to make them easier to see.  Has anyone done this?  Or do you use
>> some other type of marking dye for this purpose?
>> Thanks for your input!
>> Rebecca
>>
>> Rebecca Ashley
>> Histotechnologist
>> Wyoming State Vet Lab
>> 1174 Snowy Range Rd.
>> Laramie, WY 82070
>> Phone: 307-766-9946
>> Fax: 307-721-2051
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

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