[Histonet] Histonet Digest, Vol 151, Issue 18

Jo-Ann Bader, Ms. jo-ann.bader at mcgill.ca
Tue Jun 21 15:30:54 CDT 2016


We are happy the ABCAM CD3


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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Sent: June 21, 2016 12:00:02 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 151, Issue 18

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Today's Topics:

   1. CD3 antibody for FFPE mouse tissue (Connolly, Brett M)
   2. Re: CD3 antibody for FFPE mouse tissue (Elizabeth Chlipala)
   3. Re: CD3 antibody for FFPE mouse tissue (Connolly, Brett M)


----------------------------------------------------------------------

Message: 1
Date: Tue, 21 Jun 2016 09:59:58 -0400
From: "Connolly, Brett M" <brett_connolly at merck.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:
        <C01C35B84DCDCE49BC60867E87F1C8FE013AA4BF296B at USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations....

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly at merck.com<mailto:brett_connolly at merck.com>
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.


------------------------------

Message: 2
Date: Tue, 21 Jun 2016 09:19:49 -0600
From: Elizabeth Chlipala <liz at premierlab.com>
To: "Connolly, Brett M" <brett_connolly at merck.com>,
        "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:
        <14E2C6176416974295479C64A11CB9AE02BEC711583D at SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Brett

Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for a CD3 that only detects mouse?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz at premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Tuesday, June 21, 2016 7:59 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] CD3 antibody for FFPE mouse tissue

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations....

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly at merck.com<mailto:brett_connolly at merck.com>
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
_______________________________________________
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Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 3
Date: Tue, 21 Jun 2016 11:53:16 -0400
From: "Connolly, Brett M" <brett_connolly at merck.com>
To: Elizabeth Chlipala <liz at premierlab.com>,
        "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:
        <C01C35B84DCDCE49BC60867E87F1C8FE013AA4BF2A1B at USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"

Liz,
As long as it detects mouse T-cells it's OK. Unfortunately we have a problem getting Dako products through our ordering system.
Brett

-----Original Message-----
From: Elizabeth Chlipala [mailto:liz at premierlab.com]
Sent: Tuesday, June 21, 2016 11:20 AM
To: Connolly, Brett M; histonet at lists.utsouthwestern.edu
Subject: RE: [Histonet] CD3 antibody for FFPE mouse tissue

Brett

Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for a CD3 that only detects mouse?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz at premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Tuesday, June 21, 2016 7:59 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] CD3 antibody for FFPE mouse tissue

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations....

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly at merck.com<mailto:brett_connolly at merck.com>
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.




------------------------------

Subject: Digest Footer

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------------------------------

End of Histonet Digest, Vol 151, Issue 18
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