[Histonet] CD3 antibody for FFPE mouse tissue

[Colleen Forster]

Brett,

I use the rabbit monoclonal CD3 from Thermo (it was a Lab Vision antibody)
with great and consistent results!

The hardest part, actually getting the antibody to me....\jeez they have
problems with orders sometimes...

Here is the information for mine:

CD3 (SP7) - cat #RM-9107-S1

I use it at 1:400, citrate retrieval, 1 hour primary incubation. I use the
Rabbit on Rodent polymer from Biocare.

Respectfully,
'
Colleen Forster

On Tue, Jun 21, 2016 at 10:53 AM, Connolly, Brett M via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Liz,
> As long as it detects mouse T-cells it's OK. Unfortunately we have a
> problem getting Dako products through our ordering system.
> Brett
>
> -----Original Message-----
> From: Elizabeth Chlipala [mailto:liz at premierlab.com]
> Sent: Tuesday, June 21, 2016 11:20 AM
> To: Connolly, Brett M; histonet at lists.utsouthwestern.edu
> Subject: RE: [Histonet] CD3 antibody for FFPE mouse tissue
>
> Brett
>
> Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking
> for a CD3 that only detects mouse?
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> liz at premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> ________________________________________
> From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu]
> Sent: Tuesday, June 21, 2016 7:59 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] CD3 antibody for FFPE mouse tissue
>
> Hi all,
>
> Looking for recommendation for an anti-mouse CD3 antibody for FFPE
> sections.
>
> I have some old Histonet messages referring to a Neomarkers RB-360 ab
> which I found through Thermo, but I would like to hear about more recent
> experiences/recommendations....
>
> Thanks as always,
> Brett
>
> Brett M. Connolly, Ph.D.
> Prin. Scientist,
> Translational Biomarkers - Imaging
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_connolly at merck.com<mailto:brett_connolly at merck.com>
> T- 215-652-2501
> F- 215-993-6803
>
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