[Histonet] Powder Picric Acid vs Liquid Picric Acid

[Rene J Buesa]

If your 1.3% picric solution is in distilled water, there is not much you can do about it.If it is in acetone 38.4 mL contains 0.5 g of picric acid to which you can add 361.6 mL of acetone to get your desired concentration.René 

    On Monday, June 13, 2016 3:24 PM, Jennifer MacDonald via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 0.5 grams in 400 mL is 0.125%.  Dilute your 1.3% tenfold and it will be 
close enough.



From:  Amy Johnson via Histonet <histonet at lists.utsouthwestern.edu>
To:    "histonet at lists.utsouthwestern.edu" 
<histonet at lists.utsouthwestern.edu>
Date:  06/13/2016 10:47 AM
Subject:        [Histonet] Powder Picric Acid vs Liquid Picric Acid



Our current procedure for the Brown and Brenn Gram stain uses 0.5 grams of 
picric acid to 400mls of acetone........is there a way to use a 1.3% 
Picric Acid solution in place of the powdered Picric Acid?

Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401
715-847-2130

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