[Histonet] P16

[Cartun, Richard]

We purchase the antibody from Ventana and run it on our Bond Max platforms.  During re-optimization, we found we could dilute it 1:10 (15 minutes) following "H2" (high pH) retrieval and still get excellent results.

Richard

Richard W. Cartun, MS, PhD
Director, Histology and the Martin M. Berman, MD Immunopathology and Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Department of Pathology and Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax

________________________________________
From: Dessoye, Michael via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Wednesday, June 08, 2016 1:14 PM
To: Paula; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] P16

Ventana/Roche bought out MTM which had the patent, so they're the only vendor you can get it from.  Since you're using Bond you obviously don't want it in a dispenser, but you can buy their manual staining kit and just use the antibody from that.

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484


-----Original Message-----
From: Paula [mailto:plucas at biopath.org]
Sent: Wednesday, June 08, 2016 12:14 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] P16

Hello,

Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from.

Thanks in advance for your help

Paula

Lab manager for BioPath Medical Group in California




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