[Histonet] PFA Fixed tissue not sticking to slides?

Hobbs, Carl carl.hobbs at kcl.ac.uk
Sun Jul 31 14:06:29 CDT 2016

No...never add sucrose to "PFA" when fixing tissues for freezing....or any other time.
Fix, then sucrose- immerse until tissue sinks to bottom of receptacle.... if you can't snap-freeze effectively 

Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
SE1 1UL 
020 7848 6813    

More information about the Histonet mailing list