[Histonet] PFA Fixed tissue not sticking to slides?

[Elizabeth Chlipala]

Are you using a good plus slide?  We have started using these Tru-Bond slides when we have tissue adherence problems, they seem to work really well.  How are you handling the slides once you section?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: jenny sandy via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Friday, July 29, 2016 1:48 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] PFA Fixed tissue not sticking to slides?

Dear HistoFolks,

Has anyone had experience with cryo-sectioned tissue that is PFA fixed not adhering to slides?

The tissue is rabbit cornea culture, 4% PFA fixed O/N, then run through 20% sucrose gradient, then frozen in OCT.

We think that the PFA fix is much longer than needed for a tissue this thin, and were going to try 2 hr fix. Would over-fixation cause the section to not adhere?  

The PFA/Sucrose fixation really improves the morphology and the IHC stains that we have run so far.

Thank you in advance for your help.

Jennifer Eveleth
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