[Histonet] [External] Crystals in PAS from Ventana

[Bitting, Angela K.]

Hi Mark,
 We still have this issue after two years of complaining to Roche.  We've had multiple TAS's come in and we've sent slides to Tucson. They've gone over the instruments, etc. Long story short, their fix was adding something to their package insert that says "Schiff's Reagent is known to contain some needle-like precipitate. At expected levels, this precipitate should not affect assay performance". Good luck.

-----Original Message-----
From: Mark, Danielle via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, July 20, 2016 12:57 PM
To: histonet at lists.utsouthwestern.edu
Subject: [External] [Histonet] Crystals in PAS from Ventana

I am wondering if anyone else has been having issues with the PAS stain.  The crystals are not rinsing off of the slide using the benchmark special stainer.  We are on our 3rd lot number, no change.  We have had our tubing changed and our instrument checked to make sure the rinse is appropriate.  This problem is causing our lab great frustration.  Any feedback would be appreciated.


Danielle Mark, HT(ASCP), Technical Specialist ACL Laboratories
8901 W Lincoln Ave. | West Allis, WI 53227
O: 414.328.6235 | F: 414.328.6237 |
Danielle.mark at aurora.org<mailto:Danielle.mark at aurora.org>

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