[Histonet] 360 video of specimens

Steve McClain SteveM at mcclainlab.com
Mon Jul 18 06:19:17 CDT 2016


The next 3 weeks we are turning our lab cameras and our specimens toward making a 360 photographic orbits around specimens to create a 360 stitched image or video. 
If you know any pathologists who have tried 360 imaging around a specimen, kindly send me their name. I have not seen any prior art.

Steve A. McClain, MD

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>   1. plastic sectioning advice requested (daniel blackburn)
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> Date: Sat, 16 Jul 2016 19:51:23 +0000 (UTC)
> From: daniel blackburn <dblackburn2000 at yahoo.com>
> To: <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] plastic sectioning advice requested
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> My lab hopes to get into plastic sectioning.  We need to be able to process tissue pieces as large and thick as possible, but see that the largest embedding molds for JB4 are only 13x19mm by 5mm deep.   We have two questions:  (1) Do any of the available media (plastic or resins) allow one to embed and section large  pieces (for example eggs with dimensions of 2 cm or larger)?   (2) Is a special microtome (such as a retracting microtome) needed?   Our reason for considering plastic is that we must section yolk, which splits out of standard paraffin during sectioning. Any advice is appreciated. -- Daniel Blackburn, Trinity College 
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