[Histonet] Cleaning Tissue Molds (Kennedy, Lisa

Thu Jul 14 14:12:01 CDT 2016

Lisa,

I have used all of the previously mentioned methods for cleaning molds.  It really depends on the facilities that you have access to.  Boiling in hot soapy water is great for deep cleaning, but you may not have enough pots or beakers to clean them all at once.  Xylene, followed by 100% is good, but you may not have it in the budget to use fresh xylene and forget to save some used.  Sometimes having a container big enough to do this is also a challenge.  Spraying them down with mold release after air drying, but then you have to remember to allow them to dry.  Placing them in the processor can clog the lines, but if you place them in the oven first to drain and only do a few at a time that can help.
We have our students boil them and then dry them in the oven. Weekend dry in the oven, and on Monday am they shake them up and put them away.
Some techs don't like the residue of mold release.  It doesn't bother me though.  
Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481
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Message: 1
Date: Thu, 14 Jul 2016 10:18:12 +0000
From: "Kennedy, Lisa" <LisaKennedy at catholichealth.net>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Cleaning Tissue Molds
Message-ID:
	<517C2A781E654047A5EC98D17390F9F25D939672 at CHIEX003.CHI.catholichealth.net>

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Dear Fellow Histo Techs,
What is the BEST practice for cleaning the paraffin block tissue molds?  We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor.
Thanks so much for your help! In advance.
Sincerely,
Lisa Kennedy, HT (ASCP)

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Message: 2
Date: Thu, 14 Jul 2016 07:56:43 -0400
From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
To: "'Kennedy, Lisa'" <LisaKennedy at catholichealth.net>
Cc: "Histonet at lists.utsouthwestern.edu"
	<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cleaning Tissue Molds
Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E60B at isexstore03>
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We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30 minutes( with occasional stirring to clear the xylene), then rinse in H2O, let dry(laid out) and spray with the mold release.

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com
www.parrishmed.com

Message: 3
Date: Thu, 14 Jul 2016 13:35:22 +0000
From: <idimitro at mun.ca>
To: <Valerie.Hannen at parrishmed.com>, <LisaKennedy at catholichealth.net>
Cc: <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cleaning Tissue Molds
Message-ID:
	<14C3108E8B98EF43B3EDAE58336700340202495965 at exchange.med.mun.ca>
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We used to do that until 5 years ago, when we started looking in cleaning the molds without the use of Xylene. We found that we don't need to clan them, we turn them down on a metal tray covered with paper towel and put them in our 60 degree C oven overnight. That gets rid of the wax left on the molds. Then the next morning we spray them with mold release and they are ready for use.
 Now, bear in mind we do only research, so I don't know if you can do this in a hospital, there may be possible contamination or other issues, but it perfect solution for us.

I. Dimitrova, MLT, LHP, B.Tech., M.Sc. 

Histology Supervisor
Faculty of Medicine
Memorial University of Newfoundland
St. John's, NL Canada 

-----Original Message-----
From: Hannen, Valerie via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: July-14-16 9:27 AM
To: 'Kennedy, Lisa'
Cc: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Cleaning Tissue Molds

We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30 minutes( with occasional stirring to clear the xylene), then rinse in H2O, let dry(laid out) and spray with the mold release.

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com
www.parrishmed.com

-----Original Message-----
From: Kennedy, Lisa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 14, 2016 6:18 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cleaning Tissue Molds

Dear Fellow Histo Techs,
What is the BEST practice for cleaning the paraffin block tissue molds?  We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor.
Thanks so much for your help! In advance.
Sincerely,
Lisa Kennedy, HT (ASCP)

Message: 4
Date: Thu, 14 Jul 2016 14:11:18 +0000 (UTC)
From: Rene J Buesa <rjbuesa at yahoo.com>
To: "Kennedy, Lisa" <LisaKennedy at catholichealth.net>,
	"histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cleaning Tissue Molds
Message-ID:
	<1673797881.3661474.1468505478591.JavaMail.yahoo at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Place your molds in a 2% dishwasher soap boiling solution for 5 minutes ? was in running water for 5 minutes ? dry in a convection oven at 60?C for 10 minutes and your molds will be ready to use.As a "release" solution use a mixture 1:1 of 2-propanol and mineral oil (light weight).Ren? 

    On Thursday, July 14, 2016 6:34 AM, "Kennedy, Lisa via Histonet" <histonet at lists.utsouthwestern.edu> wrote:

 Dear Fellow Histo Techs,
What is the BEST practice for cleaning the paraffin block tissue molds?? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor.
Thanks so much for your help! In advance.
Sincerely,
Lisa Kennedy, HT (ASCP)

This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
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The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems.