[Histonet] Cleaning Tissue Molds

[idimitro at mun.ca]

We used to do that until 5 years ago, when we started looking in cleaning the molds without the use of Xylene. We found that we don't need to clan them, we turn them down on a metal tray covered with paper towel and put them in our 60 degree C oven overnight. That gets rid of the wax left on the molds. Then the next morning we spray them with mold release and they are ready for use.
 Now, bear in mind we do only research, so I don't know if you can do this in a hospital, there may be possible contamination or other issues, but it perfect solution for us.

I. Dimitrova, MLT, LHP, B.Tech., M.Sc. 

Histology Supervisor
Faculty of Medicine
Memorial University of Newfoundland
St. John's, NL Canada 

-----Original Message-----
From: Hannen, Valerie via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: July-14-16 9:27 AM
To: 'Kennedy, Lisa'
Cc: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Cleaning Tissue Molds

We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30 minutes( with occasional stirring to clear the xylene), then rinse in H2O, let dry(laid out) and spray with the mold release.

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com
www.parrishmed.com




-----Original Message-----
From: Kennedy, Lisa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 14, 2016 6:18 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cleaning Tissue Molds

Dear Fellow Histo Techs,
What is the BEST practice for cleaning the paraffin block tissue molds?  We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor.
Thanks so much for your help! In advance.
Sincerely,
Lisa Kennedy, HT (ASCP)

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