[Histonet] curiosity may kill me

Rene J Buesa rjbuesa at yahoo.com
Wed Jul 6 09:15:38 CDT 2016

I always used Auramine at room temperature to identify TB bacilli in tissue sections with fluorescence filter, never used heat and the results were as expected. Bancroft is the one describing the procedure using heat for auramine/rhodamine procedure but auramine alone at room temperature is enough.René 

    On Tuesday, July 5, 2016 4:24 PM, "Bitting, Angela K. via Histonet" <histonet at lists.utsouthwestern.edu> wrote:

 I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp.  It is because "it's always been done that way"?
Thanks group,

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