[Histonet] Clearance Angle on Microtome Blade

[Rene J Buesa]

The type of tissue, the speed of sectioning, the knife bevel and the type of paraffin (melting point) influence the clearance angle.Anywhere from 5 to 10º (preferable 5-6º) are the most used.René 

    On Friday, January 29, 2016 3:17 PM, Kelli Goodkowsky via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 Good afternoon,
Can someone touch base with me regarding the clearance angle of the microtome blade?  How would you describe a blade clearance angle that is too great or too slight, and what microtomy problems are you likely to see with each?  There seems to be conflicting information out there (or at least varying perspectives).

Thank you!
Kelli

Kelli Goodkowsky, M.Ed., HT (ASCP)
Director Clinical Education
Histologic Science
Goodwin College
(860) 727-6917
kgoodkowsky at goodwin.edu<mailto:kgoodkowsky at goodwin.edu>
http://www.goodwin.edu/






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