[Histonet] Nuclear bubbling

[Rene J Buesa]

"Nuclear bubbling", manifested as round unstained areas in the nucleus, is caused by incomplete dehydration of the section before staining. There is a review on the subject that I cannot find at this moment.René 

    On Friday, January 29, 2016 10:42 AM, "Vickroy, James via Histonet" <histonet at lists.utsouthwestern.edu> wrote:
 

 
Traditionally we regard nuclear bubbling as incomplete fixation however I'm not so sure that nuclear bubbling can't be caused by additional processing problems.  This morning I have some GI biopsies that fixed for nearly 18 hrs that have a large amount of nuclear bubbling.  We run the biopsies on a "short run".  I am wondering if possibly we are not getting rid of all of the water and therefore our dehydration steps are not long enough?

Anybody have an idea besides incomplete fixation?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvickroy at SpringfieldClinic.com<mailto:jvickroy at SpringfieldClinic.com>



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