[Histonet] unsolved proplem

[Linda]

Hello,
Did you call the vendor/manufacturer of your chemicals?  Sometimes, if they have to dispense the chemicals from a different place it can cause issues.  I had that happen with reagent alcohols.
Good luck,
Linda Dee, BGS, HT(ASCP)
 

      From: Jay Lundgren via Histonet <histonet at lists.utsouthwestern.edu>
 To: Jennifer MacDonald <JMacDonald at mtsac.edu> 
Cc: "Histonet at lists.utsouthwestern.edu" <Histonet at lists.utsouthwestern.edu>
 Sent: Thursday, January 28, 2016 12:46 PM
 Subject: Re: [Histonet] unsolved proplem
   
What kind of processor are you using?

On Tue, Jan 26, 2016 at 1:36 PM, Jennifer MacDonald via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> There are a couple of things that it might be.
> 1.  Uneven deparaffinization before staining.
> 2.  Water in your last reagents/paraffin on the processor.
>
>
>
> From:  mohamed abd el razik via Histonet
> <histonet at lists.utsouthwestern.edu>
> To:    "Histonet at lists.utsouthwestern.edu"
> <Histonet at lists.utsouthwestern.edu>
> Date:  01/26/2016 11:33 AM
> Subject:        [Histonet] unsolved proplem
>
>
>
> Dear allI have submitted a proplem about reprocessing tissue blocks that
> have patches of stained areas and unstained pale yellow areas (H&E
> stain)!!!, unfortently the proplem didn't solved yet and i have tried all
> possible solutions, increasing processing time and ordered new chemicals
> from other soruces and changed the paraplast company but still have the
> proplem !!!!
> i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each
> for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl
> benzoat 2hs at least then paraffin1+2 (Maccormick melting degree
> 56-58)each 90 mins for mice kidney, liver and tests
> the previous protocol was very satisfactory to us and we have tried to
> stain older sections processed befor the emerging proplem and have a nice
> stain by current H&E stain to ensure that the proplem is out our staining
> step.
> any suggestions please !!
> Mohamed
>
>
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