[Histonet] SOX 10 Cellmarque

[Jeffrey Robinson]

Hi Edison-  I have had some headaches getting the SOX-10 to run in the past but I now have all of the kinks ironed out and it works quite well.  I had started with the CellMarque mouse monoclonal of their RTU SOX-10.  I had it working well and then Leica recalled their red detection kits.  I could never get the mouse monoclonal to work again after that with the new red detection kits.  BioCare came out with a RTU rabbit monoclonal so I tried that and Bingo!- great staining once again.  CellMarque has since come out with their own RTU rabbit monoclonal.  They sent me a sample to try and that also stained well but I had already validated the BioCare antibody so I have stayed with that.  I run it with ER2 for 30 minutes with the red kit.  A word of caution:  do not delay when taking those slides off the Bond.  Take them off as soon as they are finished, rinse in DI water for a short period of time, run down through the alcohols (I use one 95% and 2 100% ETOH), into the xylene and coverslip immediately.  The red stain will "wash" out if you delay running the slides down.  This cannot be repaired to the best of my knowledge- you will have to rerun new slides.  I use this same protocol for Melan A and it also works great.  I use the Leica Melan A (RTU).  Good Luck!

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-----Original Message-----
From: Edison Narvaez via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, January 28, 2016 6:13 AM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] SOX 10 Cellmarque

Hello,
Anybody running Cellmarque's RTU SOX 10 antibody on the bond III, any protocol suggestions, I would like to run this antibody with Leica's red kit.


Thank you



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