[Histonet] unsolved proplem
mohamed abd el razik
k84as at yahoo.com
Tue Jan 26 13:32:37 CST 2016
Dear allI have submitted a proplem about reprocessing tissue blocks that have patches of stained areas and unstained pale yellow areas (H&E stain)!!!, unfortently the proplem didn't solved yet and i have tried all possible solutions, increasing processing time and ordered new chemicals from other soruces and changed the paraplast company but still have the proplem !!!!
i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl benzoat 2hs at least then paraffin1+2 (Maccormick melting degree 56-58)each 90 mins for mice kidney, liver and tests
the previous protocol was very satisfactory to us and we have tried to stain older sections processed befor the emerging proplem and have a nice stain by current H&E stain to ensure that the proplem is out our staining step.
any suggestions please !!
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