[Histonet] Cryojane and H&E staining

[Jamie McGinness]

I'm having issues with H&E staining from frozen fresh identical tissue cut using standard cryostat brush technique and cryojane technique. I have noticed the following. I do snap freeze tissue at -80

1.  My slides from cryojane prepared tissue appear lighter than the same tissue cut with standard brush method in the cryostat when stained with H&E. 

2.  Tissue with cryojane method appears dehydrated (vacuoles, collagen splaying, etc) compared to the standard brush technique. Again all stained with H&E and side by side comparison of same tissue. 

Thanks for any thoughts or ideas. 

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