[Histonet] IHC with H & E staining

Marcus Green marcus.green at oncology.ox.ac.uk
Wed Jan 6 05:26:35 CST 2016

Dear Users,

Happy New year - I hope this finds everybody well!

I was asked a very simple question yesterday - why don't you do H&E counterstaining on DAB stained samples. The question came about as we're looking at CD31 staining for blood-vessels and some look ruptured (we're keen to see red blood cells).

Instead of doing a consecutive cuts, a CD31 stain (Haem counterstain) on one slide and H&E on another, the question was asked why not do the Eosin stain as part of the counterstain....

I've never seen it done in the literature or in the clinic, and I've never asked why it's not done. Any assistance or advice would be greatly welcome - and my sincerest apologies if this is a very basic question?

Thanks in advance for your time,

kind regards,


Department of Oncology - University of Oxford,
Old Road Campus Research Building,
Roosevelt Drive,

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