[Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

[Simmons, Christopher]

Double PPE if you use DMSO


Sent from my iPhone

> On Feb 26, 2016, at 2:41 PM, Jay Lundgren via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> A little alcohol will lower the surface tension of aqueous solutions.  Then
> there's always DMSO.
> 
> 
>                   Sincerely,
> 
>                        Jay A. Lundgren, M.S., HTL (ASCP)
> 
> On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> 
>> Hello Histonetetrs:
>> 
>> I am trying to fix insect wings for TEM. Less than ideal fixation appears
>> to be caused by abundant setae on the surface of the wings. Those setae,
>> trap air, thus making penetration of the fixative more difficult difficult.
>> Interestingly, also the wings tend to curl upon themselves.
>> 
>> Options re. fixation:
>> 1. Just submerging the insects mechanically does not seem to solve the
>> problem.
>> 
>> 2. We are contemplating submerging and evacuating (intermittently) a few
>> times for 2 minutes (vacuuming)
>> 
>> 3. Are there chemicals that can break the bubbles formed in between the
>> setae?
>> 
>> 4. Any other comments or suggestions?
>> 
>> Options to minimize (ideally eliminate) curling of the wings?
>> 
>> If you have any constructive suggestions, please kindly email it to me
>> directly.
>> 
>> blayjorge at gmail.com
>> 
>> Of course, we intend to begin with new freshly collected insects.
>> 
>> Gratefully,
>> 
>> Jorge
>> 
>> Jorge A. Santiago-Blay, PhD
>> blaypublishers.com
>> 
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>> 
>> http://blayjorge.wordpress.com/
>> http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
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