[Histonet] auto-fluorescence

[Gayle Callis]

Dear Joost, 

Yes and to read up on this, go to this website for an excellent, well
referenced free pdf i.e. Autofluorescence, causes and cures.
https://www.google.com/?gws_rd=ssl#q=autofluorescence+causes+and+cures+wrigh
t+cell+imaging+facility   They also posted a pdf on mounting medias for
fluorescence microscopy work.  

The pdf will tell you what tissue and cellular components autofluoresce.
The authors also provided various methods to reduce or remove
autofluorescence of these components but also how to remove aldehyde induced
autofluorescence if you are fixing tissues with NBF or PFA.   

Remember there is no autofluroescence in the Near Infra Red region so one
can use an NIR fluorophore, i.e. Alexa 750 (red) or another fluorophore for
the NIR region,  and even use tissue autofluorescence as a "counterstain
fluorescence" However, you cannot see these with the human eye but these
photograph beautifully.  OR if you have a spectral imaging or confocal
capabilities,  you can rule out autofluorescence without having to treat the
tissue section.    

If you have difficulty access this pdf, I will send via private email.  

Gayle Callis HTL/HT/MT(ASCP)  
Bozeman MT USA



-----Original Message-----
From: Bruijntjes, J.P. (Joost) via Histonet
[mailto:histonet at lists.utsouthwestern.edu] 
Sent: Friday, February 26, 2016 3:00 AM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] auto-fluorescence

Hi histonetters

I hope one of you can help me. I don't have any experience with
fluorescence.

We are searching for some components and we will use  an alexa488 conjugated
secondary. Will there be an auto-fluorescence on different organs
(brain/spleen/liver)?

Greetings
Joost



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