[Histonet] Suggestions for staining bacteria in FFPE decaled growth plate

[Anna Huntley Coffey]

Happy Friday, Histonetters!

I have a puzzle that I could really use your expert knowledge on this
morning. One of our pathologists has a decalcified FFPE mouse bone embedded
longitudinally and has found some bacteria located in the growth plate. The
bacteria has been confirmed with fluorescence, but she really wants to be
able to show the morphology of the tissue with the bacteria. So far, H&E,
gram, Brown & Hopps stain the growth plate so dark that you can't make out
the bacteria. My suggestions were to try a thinner section and adjust the
timing of the H&E (haven't had a chance to do that yet), but I wanted to
throw the question out to you all to see if anyone has tried other stains
or techniques for this type of issue.

I'd really appreciate the help!

Thanks,
Anna