[Histonet] Question re: accessory piece for tissue flotation bath

[Manfre, Philip]

With regards to the HistoOrientator, it does removes wrinkles from the sections fairly effectively.  That being said, I would not use it on any sections intended for immunohistochemistry.  The hot plate on this device gets very hot and would no doubt damage or affect the antigenicity of such tissues.  Also, some practice is required to effectively use it.  If you do not drain the slides somewhat first, the tissues will quickly move all over the slide as they heat.  They will move quite a bit, sometimes butting up against each other.  If you drain them too long, then the tissues start to dry onto the slide, wrinkles and all.  So, as I said, spend some time practicing with this device with practice tissue until you get the hang of it.  It is a good tool that is tricky to use at first.

Good luck!
Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre at merck.com



-----Original Message-----
From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, February 24, 2016 8:36 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Question re: accessory piece for tissue flotation bath

Morning!

Can anyone of you share the functionality of:

Flotation Work Station w/ 8" x 8" x 2 1/4" (Deep Dish), HistoOrientator and Dryer

Curious if the HistoOrientator actually removes wrinkles as the description states.

Thanks!

Jeanine H. Sanders, BS, HT (ASCP), QIHC
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS/G-32
Atlanta, GA 30329
jqb7 at cdc.gov<mailto:jqb7 at cdc.gov>
404-639-3590

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