[Histonet] Shrinkage

[Colleen Forster]

I find the same as Caroline Miller, about 20-25% with formalin fixation and
routine processing.

C

On Mon, Feb 22, 2016 at 3:41 PM, Caroline Miller via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> hi All, I have done some experiments in this area for mouse brains, and I
> find that there is actually an expansion of tissue after formalin fixation
> (around 10%), but then certainly a shrinkage to 100% dehydration agent of
> about 20% from the original size. We found similar results with alcohol,
> acetone and THF.
>
> This is manual fluid changes or a day per solution, with no vacuum, temp or
> pressure.
>
> Happy to share the data if anyone is interested
>
> yours,
> mills
>
> On Mon, Feb 22, 2016 at 1:10 PM, Terri Braud via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > LOL...Shrinkage...heh, heh.
> > But seriously, there should be little to no gross shrinkage from formalin
> > fixation and if the specimen is properly fixed, then there should be very
> > little gross shrinkage as it is dehydrated.  That is supposed to be the
> > point!  If someone is getting 30% shrinkage, there is something seriously
> > wrong with their processing schedule.
> > Sincerely, Terri
> > Terri L. Braud, HT(ASCP)
> > Anatomic Pathology Supervisor
> >
> > Today's Topics:
> >    2. formalin and shrinkage (Gudrun Lang)
> >
> >
> > Message: 2
> > Date: Mon, 22 Feb 2016 16:59:21 +0100
> > From: "Gudrun Lang" <gu.lang at gmx.at>
> > Subject: [Histonet] formalin and shrinkage
> > Hi!
> > Today someone asked me about shrinkage caused by the fixation with
> > formaldehyde specially on skin-biopsies.  She spoke about shrinkage of
> 30%
> > percent. In my opinion shrinkage is mainly caused by the processing with
> > dehydration and defatting. Formaldehyde renders the tissue harder but not
> > strictly smaller.
> > What is the opinion of the community?
> > Gudrun
> >
> >
> >
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>
>
>
> --
> Caroline Miller (mills)
> Director of Histology
> 3Scan.com
> 415 2187297
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