[Histonet] keratohyalin granules

[Gudrun Lang]

Do you stain with hematoxylin only? or also with eosin. I think eosin is the
dye, that would highlight the basic proteins in the granula.
Gudrun

-----Ursprüngliche Nachricht-----
Von: Kalleberg, Kristopher via Histonet
[mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Mittwoch, 17. Februar 2016 21:37
An: histonet at lists.utsouthwestern.edu;
histonet-request at lists.utsouthwestern.edu
Betreff: [Histonet] keratohyalin granules

Hello All,

Does anyone have experience staining the keratohyalin granules in skin with
different types of hematoxylins?  I am currently looking at keratohyalin
granules and staining with Harris hematoxylin and there seems to be little
staining and not as prominent as I would have expected.  Wonder if anyone
has noticed that different hematoxlins stain keratohyalin granules better
than others.  The skin is normal skin photoprotected and photoexposed.
Thank you in advance for any helpful insights.

Kris
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