[Histonet] Nuclear Bubbling

Tue Feb 16 16:18:03 CST 2016

I don't have your answer Jim, yet I can vouch for your experience as a
supervisor since you were my boss for about two years.  Hopefully you can
get it resolved because it can be very frustrating as the tech.

On Tuesday, February 16, 2016, Vickroy, James via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> For the record please note that I have over thirty-six years experience
> working in a Histology lab.   I have been a supervisor or manager  of a
> hospital and clinic histology department for at least 25 years.
>
> Jim Vickroy
> Histology Manager
> Springfield Clinic, Main Campus, East Building
> 1025 South 6th Street
> Springfield, Illinois  62703
> Office:  217-528-7541, Ext. 15121
> Email:  jvickroy at SpringfieldClinic.com<mailto:
> jvickroy at SpringfieldClinic.com <javascript:;>>
>
>
> From: Jamal Rowaihi [mailto:j.rowaihi at alborglaboratories.com
> <javascript:;>]
> Sent: Tuesday, February 16, 2016 1:56 PM
> To: Manfre, Philip; Rene J Buesa; Vickroy, James
> Cc: جمال الرويحي; histonet at lists.utsouthwestern.edu <javascript:;>
> Subject: Re: [Histonet] Nuclear Bubbling
>
> Great, I agree
>
>
>
> Regards
>
> Jamal Rowaihi
> Anatomic Pathology Supervisor
> Al Borg Medical Laboratories
> Sent from my cell phone
> -------- Original message --------
> From: "Manfre, Philip via Histonet" <histonet at lists.utsouthwestern.edu
> <javascript:;><mailto:histonet at lists.utsouthwestern.edu <javascript:;>>>
> Date: 2/16/2016 10:44 PM (GMT+03:00)
> To: Rene J Buesa <rjbuesa at yahoo.com <javascript:;><mailto:
> rjbuesa at yahoo.com <javascript:;>>>, "Vickroy, James"
> <jvickroy at SpringfieldClinic.com<mailto:jvickroy at SpringfieldClinic.com
> <javascript:;>>>
> Cc: histonet at lists.utsouthwestern.edu <javascript:;><mailto:
> histonet at lists.utsouthwestern.edu <javascript:;>>
> Subject: Re: [Histonet] Nuclear Bubbling
>
> Sort of a rude response to someone looking for help.
>
> -----Original Message-----
> From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu
> <javascript:;>]
> Sent: Tuesday, February 16, 2016 1:12 PM
> To: Vickroy, James; histonet at lists.utsouthwestern.edu <javascript:;>
> <mailto:histonet at lists.utsouthwestern.edu <javascript:;>>
> Subject: Re: [Histonet] Nuclear Bubbling
>
> If I remember correctly, this issue has been discussed previously.The
> general consensus as to the cause of nuclear "bubbling" (in reality a lack
> of staining in the nuclear area) has been attributed to an incomplete
> section drying.After the section has be "fished" from the water bath, if
> the slide is not set to drain the underneath water before drying, the
> nuclear components are dissolved hence when the section is stained, there
> is nothing to stain → "nuclear bubbling".I think this has been previously
> stated so I really do not understand posting this same question again.I do
> not think that posting again the question a different answer is going to be
> received.rené
>
>     On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <
> histonet at lists.utsouthwestern.edu <javascript:;><mailto:
> histonet at lists.utsouthwestern.edu <javascript:;>>> wrote:
>
>
>
> Struggling to find an answer.  We do a lot of GI biopsies in our lab.
> Sometimes they look wonderful without any nuclear bubbling, other times the
> bubbling is pretty intense.  Since nuclear bubbling is often attributed to
> incomplete fixation we of course have investigated the fixation times.  I
> do not find that the problem is fixation.  In fact some of the biopsies end
> up fixing for 48 hrs before processing. (weekend).  There was a suggestion
> last week or so that there might be water trapped under the slides after
> cutting and before staining.  I really thought that this might be the issue
> however I'm not sure at this point.  Extra drying seems to help but
> sometimes slides side by side are so variable, one with bubbles and one
> without.  I also don't believe the problem is in the processing schedule
> since the problem has shown up on both a rapid and a normal schedule.
> (therefore longer dehydration, clearing, etc.)
>
> I am wondering if anyone else has worked with this issue.  Here are my
> questions:
>
>
> 1.        Could it be something that is happening with the tissue before
> it gets to the lab?  Usually a delay if fixation  causes other artifacts
> but not bubbling.  Could it be heat from the GI procedure?
>
> 2.      We do use blue sponges for our biopsies.  I know some say get rid
> of the sponges but has anyone seen this problem caused by usage of sponges?
>
> 3.      What about the heat stage in our Prisma stainer?
>
>
> I am really getting frustrated.  Pathologists never complain however I
> would rather all of the tissue did not have the "nuclear bubbling".  Again
> we only do biopsies so I really don't think the standard old " not enough
> time in formalin" is the issue.  I have even wondered about variables such
> as we use recycled formalin, recycled Clearite III.
>
> Any suggestions?
>
> Jim
>
>
>
> Jim Vickroy
> Histology Manager
> Springfield Clinic, Main Campus, East Building
> 1025 South 6th Street
> Springfield, Illinois  62703
> Office:  217-528-7541, Ext. 15121
> Email:  jvickroy at SpringfieldClinic.com<mailto:
> jvickroy at SpringfieldClinic.com <javascript:;><mailto:
> jvickroy at SpringfieldClinic.com <javascript:;>
> %3cmailto:jvickroy at SpringfieldClinic.com>>
>
>
>
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-- 
Katie L. Sands
Histology Technician/Lab Manager
Advanced Dermatology of Southeast Missouri, PC
2116 Megan Drive Suite 102
Cape Girardeau, MO  63701
derm.katiesands at gmail.com
Work:  (573) 335-7546
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