[Histonet] Nuclear Bubbling

[Jennifer MacDonald]

There is no need to be rude.  He has tried the drying option and is still 
having nuclear bubbling.  He is exploring other possible issues.  You 
would see this if you read the email in its entirety. 



From:   Rene J Buesa via Histonet <histonet at lists.utsouthwestern.edu>
To:     "Vickroy, James" <jvickroy at SpringfieldClinic.com>, 
"histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu>
Date:   02/16/2016 10:13 AM
Subject:        Re: [Histonet] Nuclear Bubbling



If I remember correctly, this issue has been discussed previously.The 
general consensus as to the cause of nuclear "bubbling" (in reality a lack 
of staining in the nuclear area) has been attributed to an incomplete 
section drying.After the section has be "fished" from the water bath, if 
the slide is not set to drain the underneath water before drying, the 
nuclear components are dissolved hence when the section is stained, there 
is nothing to stain → "nuclear bubbling".I think this has been previously 
stated so I really do not understand posting this same question again.I do 
not think that posting again the question a different answer is going to 
be received.rené 

    On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" 
<histonet at lists.utsouthwestern.edu> wrote:
 

 
Struggling to find an answer.  We do a lot of GI biopsies in our lab.  
Sometimes they look wonderful without any nuclear bubbling, other times 
the bubbling is pretty intense.  Since nuclear bubbling is often 
attributed to incomplete fixation we of course have investigated the 
fixation times.  I do not find that the problem is fixation.  In fact some 
of the biopsies end up fixing for 48 hrs before processing. (weekend).  
There was a suggestion last week or so that there might be water trapped 
under the slides after cutting and before staining.  I really thought that 
this might be the issue however I'm not sure at this point.  Extra drying 
seems to help but sometimes slides side by side are so variable, one with 
bubbles and one without.  I also don't believe the problem is in the 
processing schedule since the problem has shown up on both a rapid and a 
normal schedule. (therefore longer dehydration, clearing, etc.)

I am wondering if anyone else has worked with this issue.  Here are my 
questions:


1.        Could it be something that is happening with the tissue before 
it gets to the lab?  Usually a delay if fixation  causes other artifacts 
but not bubbling.  Could it be heat from the GI procedure?

2.      We do use blue sponges for our biopsies.  I know some say get rid 
of the sponges but has anyone seen this problem caused by usage of 
sponges?

3.      What about the heat stage in our Prisma stainer?


I am really getting frustrated.  Pathologists never complain however I 
would rather all of the tissue did not have the "nuclear bubbling".  Again 
we only do biopsies so I really don't think the standard old " not enough 
time in formalin" is the issue.  I have even wondered about variables such 
as we use recycled formalin, recycled Clearite III.

Any suggestions?

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvickroy at SpringfieldClinic.com<
mailto:jvickroy at SpringfieldClinic.com>



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