[Histonet] (CLRW) Clinical Laboratory Reagent Water

Brent Adams badams at acadianagastro.com
Tue Feb 16 12:34:46 CST 2016


Deanne,

I purchase 5 liter cubes from Mercedes Medical.
They can provide test results on each batch of water and fax to you.

I did have a CLIA inspector advise to document daily on the water as is
written on the cube for clarity of water, no sediments or signs of contamination.

Think 5 Liters is about $12.50 and I use a little less than two a month.


Brent Adams – BS, LPN, HT


www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476

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Today's Topics:

   1. (CLRW) - Clinical Laboratory Reagent Water (Knutson, Deanne)
   2. Tissue cassette baskets for VIP 6 (Vickroy, James)
   3. MICROTOME KNIFE SHARPENING (Hannen, Valerie)
   4. Re: (CLRW) - Clinical Laboratory Reagent Water (Morken, Timothy)
   5. Nuclear Bubbling (Vickroy, James)
   6. Complementary webinar on post-processing scientific       images,
      Feb 24 @ 1PM EST (J. Sedgewick)


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Message: 1
Date: Mon, 15 Feb 2016 12:14:06 -0600
From: "Knutson, Deanne" <DKnutson at primecare.org>
To: "'histonet at lists.utsouthwestern.edu'"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water
Message-ID:
        <1E0E2B14C709174B8AC2BE0AE7F76833A4D5970FFC at EXCHANGE2K7.staprimecare.org>

Content-Type: text/plain; charset="us-ascii"

Fellow Histonetters,

Our medical center will no longer be providing us with water for our laboratory procedures.
I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so?

Thank you for your help!

Deanne Knutson
Supervisor
Anatomic Pathology




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Message: 2
Date: Mon, 15 Feb 2016 19:12:06 +0000
From: "Vickroy, James" <jvickroy at SpringfieldClinic.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Tissue cassette baskets for VIP 6
Message-ID:
        <9B1A1501A800064397369BD8072E6BCA06502BD8 at E2K10DB.springfieldclinic.com>

Content-Type: text/plain; charset="us-ascii"


Anybody have an idea where we can get a used cassette basket that will fit in the VIP 6?  The cost of a new one is pretty high?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvickroy at SpringfieldClinic.com<mailto:jvickroy at SpringfieldClinic.com>



This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you.

------------------------------

Message: 3
Date: Tue, 16 Feb 2016 10:28:59 -0500
From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
To: "Histonet at lists.utsouthwestern.edu"
        <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] MICROTOME KNIFE SHARPENING
Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750BC at isexstore03>
Content-Type: text/plain; charset="us-ascii"

Hi all...  Hoping you might be answer a couple of questions that I have.  1) Does anyone use a microtome knife sharpening kit made by Pathco?

If so, 2) How well does it sharpen the blades?  3) Is there any issue on attaching the abrasive sheets to the honing plate?  4) Do the abrasive sheets tend to "move"

during the sharpening process, that would cause the blade not be on them but on the plate instead?

  I currently am using coarse and fine abrasive liquids along with the honing plate... but the coarse, fine and honing liquids are becoming quite expensive and hard to come by.

Any and all replies are welcomed.

Thank you in advance,



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com<mailto:valerie.hannen at parrishmed.com>
www.parrishmed.com

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Message: 4
Date: Tue, 16 Feb 2016 16:05:12 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: "Knutson, Deanne" <DKnutson at primecare.org>
Cc: Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] (CLRW) - Clinical Laboratory Reagent Water
Message-ID:
        <761E2B5697F795489C8710BCC72141FF6FD17480 at ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"

Deanne,

If you just need it for a few critical reagents You can get type 1 water by the pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for most histology procedures, but convenient if you need it. Note that the expiry clock of 30 days starts when you open the bottle.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Monday, February 15, 2016 10:14 AM
To: 'histonet at lists.utsouthwestern.edu'
Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water

Fellow Histonetters,

Our medical center will no longer be providing us with water for our laboratory procedures.
I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so?

Thank you for your help!

Deanne Knutson
Supervisor
Anatomic Pathology




  ________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.

This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
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------------------------------

Message: 5
Date: Tue, 16 Feb 2016 17:10:40 +0000
From: "Vickroy, James" <jvickroy at SpringfieldClinic.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Nuclear Bubbling
Message-ID:
        <9B1A1501A800064397369BD8072E6BCA06503B35 at E2K10DB.springfieldclinic.com>

Content-Type: text/plain; charset="us-ascii"


Struggling to find an answer.  We do a lot of GI biopsies in our lab.   Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.  Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.  I do not find that the problem is fixation.  In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).  There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.   I really thought that this might be the issue however I'm not sure at this point.  Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.   I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.)

I am wondering if anyone else has worked with this issue.  Here are my questions:


1.        Could it be something that is happening with the tissue before it gets to the lab?  Usually a delay if fixation  causes other artifacts but not bubbling.  Could it be heat from the GI procedure?

2.       We do use blue sponges for our biopsies.  I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges?

3.       What about the heat stage in our Prisma stainer?


I am really getting frustrated.  Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".   Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.  I have even wondered about variables such as we use recycled formalin, recycled Clearite III.

Any suggestions?

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvickroy at SpringfieldClinic.com<mailto:jvickroy at SpringfieldClinic.com>



This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you.

------------------------------

Message: 6
Date: Tue, 16 Feb 2016 11:31:27 -0600
From: "J. Sedgewick" <jerrysedgewick at gmail.com>
To: <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Complementary webinar on post-processing
        scientific      images, Feb 24 @ 1PM EST
Message-ID: <1557B579CB2A478B884FD20B9E5064D6 at sedge>
Content-Type: text/plain; format=flowed; charset="UTF-8";
        reply-type=original

Hello All,

Do you post-process your scientific images?  Typical tools for common
adjustments include Photoshop and ImageJ, but they aren?t the best answer.
You are invited to attend a complementary webinar on Feb 24 at 1:00PM EST ?
?Best Practices for Post-Processing of Scientific Images?

Reserve your webinar seat now at
https://attendee.gotowebinar.com/register/1379379490730827010

I'll be giving this webinar. Also, a similar presentation will be given for
the histology WOW 2016 with Dr. Michael Linden at the University of
Minnesota on March 25.

Best,
Jerry Sedgewick






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