[Histonet] C3 IF staining of FFPE tissue (Kristyn Ferber)

[Erickson, Jamie E]

Hello, Kristyn,
                             I don't know what species you work with but I work in mouse (mouse lupus kidneys) and had good luck with Dakos C3c antibody F0201 using Dakos Proteinase K 10 minutes at room temperature.  I used a unlabeled version that is not available anymore but F0201 (FITC) one is said to be of the same clone. I had aliquot lots of the old antibody that just ran out so  I have not tried this FITC one yet.  If you want  to  stain using DAB I've used a Rabbit anti-FITC (Invitrogen) and then come back with  Anti-rabbit HRP polymer then DAB. Its works nice when you have a FITC primary but don't want to visualize with Fluorescence due to background etc..  
We have tested a lot of Ab's on paraffin for various epitopes and have a good success rate you just may have to test some antibodies and a few pretreatment conditions (EDTA, Citrate, PTK, Protease 1) we use a Leica Bond autostainer. 

Best of luck.

Jamie Erickson
Scientist
Abbvie bioresearch Center
Worcester, Ma 01605

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Subject: Histonet Digest, Vol 147, Issue 8

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Today's Topics:

   1. C3 IF staining of FFPE tissue (Kristyn Ferber)
   2. Issue with Leica/Jung Histoembedder (dayuang)
   3. RELIA Histology Careers Bulletin Special Edition for	Managers
      and Supervisors - 2-8-2016 (Pam Barker)


----------------------------------------------------------------------

Message: 1
Date: Mon, 8 Feb 2016 08:15:21 -0500
From: Kristyn Ferber <kristyn.ferber at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] C3 IF staining of FFPE tissue
Message-ID:
	<CAMnUO=bxG+f5ZyTuR2QhVt7ff3uQdhhcyTk=sJT=_Lrmj8Jvhw at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Hello List,

I suspect it may be a lost cause (since processing likely kills the antigen
site) but has anyone had any luck with IF staining for C3 on FFPE tissue? I
know frozen is ideal but occasionally, we need to revert to the FFPE
sample.

Best,

Kristyn


------------------------------

Message: 2
Date: Mon, 8 Feb 2016 11:52:28 -0500
From: dayuang <dayuang at rci.rutgers.edu>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Issue with Leica/Jung Histoembedder
Message-ID: <93D4868D-8741-4586-9F9A-0CC155D3AC3C at rci.rutgers.edu>
Content-Type: text/plain;	charset=utf-8

Dear all,

We have a Leica/Jung Histoembedder, and it stopped working twice recently during embedding. The symptoms are: illumination light went off, hot and cold plates stopped working, the wax dispenser didn?t flow anymore, wax reservoir stopped heating. There was no error message displayed. Switching the power off and on corrected the problem immediately at the first time. However for the 2nd onset, we had to wait for hours until it came back.

Please kindly share your experience if anybody has undergone and resolved the problem. I am more than grateful to your kind input.

Best regards,
Dayuan



*******************************************
Dayuan Gao, Ph.D.
Assistant Research Professor
Department of Pharmaceutics
Ernest Mario School of Pharmacy
Rutgers, The State University of New Jersey
160 Frelinghuysen Road
Piscataway, New Jersey 08854
Phone: (848)445-6837
Fax:   (732)445-3134
*******************************************








------------------------------

Message: 3
Date: Mon, 8 Feb 2016 12:44:26 -0500
From: "Pam Barker" <relia1 at earthlink.net>
To: <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition
	for	Managers and Supervisors - 2-8-2016
Message-ID: <05ce01d16298$5ac424d0$104c6e70$@earthlink.net>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Histopeeps!
What would make the perfect management position?
? Is it the location?
? Perhaps the type of lab?
? How about the size of the staff/lab?
? Maybe it?s the Hours? The Money and/or benefits?
? Could it be that a particular situation is the next logical step in your
career?

More than likely the answer is all of the above in different degrees.
That is why I am contacting you with this special bulletin.  I am starting
to get a lot of management opportunities and I wanted to touch base and let
you know.  Your next opportunity might be just around the corner and I might
have it for you.  If you are looking for a position right now please contact
me right away.  We can talk about my current positions OR about a customized
search on your behalf.  If you aren?t looking right away but want to let me
know what would make a perfect job for you so that I could keep an eye out,
that would be great too.  To do that just shoot me an email at
relia1 at earthlink.net or call me toll free at 866-607-3542.  

Here is a list of my current managerial opportunities:
Lab Manager ? Williamsburg, VA
Pathology Supervisor ? Manchester, NH
Histology Supervisor ? Amarillo, TX
Lead Tech/Supervisor ? Fayetteville, AR
Sr. Clinical Lab Specialist/Pathology ? Norfolk, VA
IHC Tech Support ? Los Angeles, CA

All of these clients offer autonomy in your position and a staff eager to
welcome their new manager.

I really appreciate you taking time out of your busy day to read my e-mail
Thanks Again!?

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
?Pam M. Barker
?
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:???? (407)353-5070
FAX:???? (407)678-2788
E-mail: relia1 at earthlink.net 
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 








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End of Histonet Digest, Vol 147, Issue 8
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