[Histonet] Wright's stain

[taylor alan]

> On 26 December 2016 at 22:15 taylor alan <aj.taylor at blueyonder.co.uk> wrote:
> 
> 
>     Hi Lauren
> 
>     With reference to your enquiry for the preparation of Wrights stain from
> Wrights stain powder
> 
>     I have found the original method of preparing Wrights stain in one of my
> favourite 'historical' pathology text books. Practical Bacteriology, Bloodwork
> and Parasitology. Eighth Edition. Edited by Rear Admiral Professor E R Stitt.
> USN. 1927. Who was the Director of the Naval Medical School in Washington and
> the Phillipines. Many older technicians and medical staff who served time in
> the Navy may remember this great text.
> 
>     Stitt details Wrights original method of preparing this stain, over a
> paragraph, cutting tothe chase the resulting dried precipitate is scraped from
> the filter paper for storage. The method for preparing a working solution is
> to weigh out 300mg of stain powder and add to this in a flask 100ml of pure
> (acetone free) methyl alcohol to dissolve.(All Romanowsky stains require this
> same alcohol).
> 
>     Attached to this paragraph is an additional recommendation by Wright to
> make a stock solution by weighing out 100mg of stain powder and dissolving
> this in 60ml of methyl alcohol, as above. For use filter off 20ml and add to
> the filtrate 5ml of pure methyl alcohol to make a working solution.
> 
>     I would be very happy to copy the whole paragraph for you, detailing the
> lengthy preparation of this stain if you wish. If you have a very aged bottle
> of this stain powder there is a very good chance that you will obtain better
> results than from a stain prepared in more recent years. We have dry powder
> stains that are more than 50 years old that give precise and beautiful
> results, these are used sparingly and are very precious to us here in our
> laboratory.
> 
>     Hoping you have success with your staining and the methodology detailed
> above is helpful to you and your colleagues.
> 
>     Best Regards
> 
>     Alan Taylor BSc(Hons), FRMS.
> 
>     Microtechnical Services
> 
>     Exeter. UK.
> 
> 
>         > > 
> >         On 22 December 2016 at 13:35 Lauren Sweeney via Histonet
> > <histonet at lists.utsouthwestern.edu> wrote:
> > 
> > 
> >         Hello all in histoland-
> > 
> >         Does anyone out there have a recipe for preparing Wright's stain
> > from powder? Our pathologist wants us to do Sheehan's modification Giemsa
> > and we don't have any commercially prepared Wrights left but we do have an
> > old bottle of Wright's powder.
> > 
> >         Happy Holidays!
> > 
> >         L
> > 
> > 
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> >     >