[Histonet] Processing of frozen material

Julio Benavides j.benavides at eae.csic.es
Fri Dec 23 11:12:12 CST 2016


Hi there,

I have frozen (-20ºC) samples  from skeletal muscle (sheep samples)  
that now requiere processing and cutting for HE and IHC. Any advice on  
how to proceed? better to put them in formalin while still frozen or  
first thaw them? A good way to thaw them preserving the most of the  
histological architecture?


Thanks a lot for your help and have a very nice Chritsmas. All the  
best for 2017!

Julio






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