[Histonet] Coverslipping

Ifeoluwa Ajayi ajayiifeoluwa at gmail.com
Wed Dec 21 23:08:40 CST 2016

After cover slipping we just allow the slide to stay on the bench for it to
dry. We then carefully remove the extra mountant with the aid of scalpel
blade. Of course dust may cover it but cotton wool and xylene this can be

Ajayi Ifeoluwa BMLS, MSc
University College Hospital UCH
Ibadan, Nigeria.

On Dec 21, 2016 10:31 PM, "Jessica Riggleman via Histonet" <
histonet at lists.utsouthwestern.edu> wrote:

> Hello,
> Would anyone mind sharing their procedure after coverslipping a slide?
> Do you let the slide sit and dry? Do you use xylenes to wipe of the extra
> mounting medium immediately?
> I noticed after storing the slides in a slide box, a lot of dust collects.
> Is this because of the xylenes? Should I wipe the slide with alcohol or
> wrap them to prevent this? What do you use to wrap your slides?
> Please keep in mind they are large, 2x3 slides.
> Thank you for all of your input,
> Best,
> Jessica Riggleman
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