[Histonet] Histonet Digest, Vol 157, Issue 13

[Mesru T]

Hi Jamie,


1. The sheets have adhesive part and holding non-adhesive part. The sheet
is as big as a regular A4 size. The length of the individual tapes is fixed
but you can cut the sheets in different widths depending on the width of
the sample. You can also order sheets with different fixed lengths.
Probably 20-30 sectioning tapes can be generated from one sheet.
2. Nobody knows the chemistry of the transfer film except the creator.
3. You can use any media if you dehydrate you can use permanent or if not
aqueous media. Their media is better optimized for the tape optics for
imaging.
4. Regular OCT does not produce as good sections. SCEM works great. SCEM L1
is new and improved product. I have not tried.
5. The 2C cryofilms are the old versions. The new and improved version is
3C(16UF). The improved version produces better sections.

Mesruh Turkekul





> I am looking into trying the Kawamoto's film method. ?I have the order
> sheet for the cryofilm sheets and other supplies. ?I did have a few
> questions.
> 1. ?I was wondering if anyone knows when you order a sheet how many slides
> can be produced from that sheet? ?2. ?Does anyone know what the transfer
> film is??3. ?What are the advantages to using their SCCM mounting medium
> ?and the difference between the four types offered G1, R1, R2, R3?4. ?What
> are the advantages of using heir SCEM embedding medium and the difference
> between the SCEM and SCEM L1? ?5. ?Does anyone know the differences of the
> cryofilm 2C(9), 2C(10), and 3C(16UF)?
> Thanks so much for any information you may have.
> Jamie??
>
>
>