[Histonet] histological processing of silicone

[Kruttwig, Klaus]

Dear colleagues,


has anyone experience with embedding and sectioning of tissue attached to silicone, e.g. PDMS? I tried cryosectioning as well as sectioning of paraffin containing thin sheets of silicone with-or without tissue. Both methods were not very encouraging. The silicone seems to be able to 'move' within the block and gets eventually disrupted after/while touching the blade. I guess, because of the missing stable connection between the silicone and the embedding medium. Another problem is swelling of the polymer in the organic solvent-but, I can deal with that.

Any help will be greatly appreciated.


Thanks,

Klaus


Klaus Kruttwig

INM-Leibniz Institute for New Materials

Saarbruecken,

Germanz

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