[Histonet] Histonet Digest, Vol 157, Issue 1

[WILLIAM JACKSON]

Can someone please give me some tips in using an automatic microtome wheel, were the advance wheel keeps movivg eith forward of backward until you take your hand off of it, any little tip is useful. Meyosha Jackson HT(ASCP)

> 
>     On December 1, 2016 at 1:00 PM histonet-request at lists.utsouthwestern.edu wrote:
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>     Today's Topics:
> 
>        1. Re: Uneven ER/PR (Terri Braud)
>        2. HCC Control Tissue (Verno, Victoria R.)
>        3. Reticulin stain for poorly processed tissue (sharon Celligent)
>        4. Histology Openings Nationwide (Angie Laparidis)
> 
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>     On November 30, 2016 at 1:38 PM Terri Braud <tbraud at holyredeemer.com> wrote:
> 
>     My first 2 items to check whenever I have uneven IHC staining are (1) Inadequate deparaffinization, (2) bad lot of charged slides - yes this can cause terribly streaky or spotty staining, and since you are using the Bond, perhaps there was an issue with coverplate placements.
>     Just things to consider. Hope this helps, Terri
> 
>     Terri L. Braud, HT(ASCP)
>     Anatomic Pathology Supervisor
>     Laboratory
>     Holy Redeemer Hospital
>     1648 Huntingdon Pike
>     Meadowbrook, PA 19046
>     ph: 215-938-3689
>     fax: 215-938-3874
> 
>     Today's Topics:
> 
>        1. ER/PR Uneven Staining (Joanne Clark)
>           Message: 2
>           Date: Tue, 29 Nov 2016 21:09:17 +0000
>           From: Joanne Clark <jclark at pcnm.com>
>           Subject: [Histonet] ER/PR Uneven Staining
>           I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND.
>           Joanne Clark, BAAS, HT(ASCP)CM
>           Director of Histology
> 
>     On December 1, 2016 at 11:31 AM "Verno, Victoria R." <Victoria_Verno at bshsi.org> wrote:
> 
>     Hey anyone out there have any HCC control tissue? We are looking for positive liver control tissue to validate Glypican-3. Thank you for your assistance.
> 
>     Victoria Verno
> 
>     Maryview Histology Supervisor
> 
>     Maryview Medical Center
> 
>     3636 High Street
> 
>     Portsmouth, VA 23707
> 
>     757-398-4763
> 
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>     On December 1, 2016 at 11:58 AM sharon Celligent <sharon.celligent at gmail.com> wrote:
> 
>     I am working on a project and have received slides containing tissue that
>     seem to be poorly processed. I am currently using a Chandler's Reticulin
>     stain kit that was purchased through American MasterTech. My liver control
>     on the test slide is working but the tissue of unknown processing is not
>     staining. The following conditions may be the problem: poor processing,
>     disease process, treatment of disease.
>     Thank you,
>     Sharon
> 
>     On December 1, 2016 at 12:53 PM Angie Laparidis <angie at ka-recruiting.com> wrote:
> 
>     Hi Histonet Members,
> 
>     I hope you are doing well. I am a Recruiter at a highly successful and well
>     respected Healthcare recruiting firm. I help place Histology Professionals
>     in permanent positions across the country and I wanted to see if you or
>     someone you know may be interested in exploring other career opportunities?
>     We are completely free of charge to candidates and and we work on quite a
>     few laboratory openings across the country. Our clients typically assist
>     with relocation expenses.
> 
>     Below is a list of some of the other opportunities we are currently working
>     on. If you do not see an opening in a location in which you live or would
>     like to live, please send me an email me a copy of your resume and let me
>     know where you would be interested in a job. I will then tailor a search
>     for you that is completely confidential and free to candidates.
> 
>     Histology Job Opportunities (HT or HTL):
> 
>     AR-Little Rock-Histotech (1st shift 4:30/5 am start)
>     FL-Tampa-HLA Supervisor
>     IL-Chicago-Histotechnician
>     ME-Southern/coastal-Histotech
>     NC-Raleigh-IHC Tech
>     NC-Raleigh-Histotech
>     ND-Fargo-Pathologists Assistant
>     NM-Southern-Assistant Histology Supervisor
>     NY-Long Island-Histotech (NYS license required)
>     NY-Long Island-Histology Supervisor
>     MO-St Louis-Histotech (3rd shift)
>     OH-Columbus-Histotech
>     TN-Nashville-Histotech
>     VA-Richmond-Histotechnologist (days)
>     VA-Roanoke-Histotech
>     WI-Green Bay-HT/ HTL (2nd & 3rd shifts)
> 
>     If you're interested in learning more about these opportunities or
>     opportunities in a certain geographic location please reply with an updated
>     resume and let me know when a good time to reach you is.
> 
>     If this is not the right fit for you please let me know who you can
>     recommend and give me an idea of what types of positions you'd be
>     interested in hearing about in the future. I cover the entire US and have
>     am working on Lab positions at all levels. We offer a very generous
>     referral bonus for anyone you refer to us that we place into any position
>     across the country.
> 
>     To view some additional opportunities please visit our website at
>     www.ka-recruiting.com.
> 
>     Sincerely,
> 
>     *Angie Laparidis*Healthcare Recruiter
>     K.A. Recruiting, Inc.
>     10 Post Office Square, 8th Floor South, Boston, MA, 02109
>     W: 617.746-2744 (*please note this is a new number*)
>     F: (617) 507-8009
>     Angie at ka-recruiting.com
> 
>     Our openings are updated daily at www.ka-recruiting.com
>