[Histonet] Modified Movat's Pentachrome stain

[Angela Lamberth]

Hi Histonetters!

I’m gearing up to perform a pentachrome stain. I will be making this in
house and not using a kit. Through searching histonet, I’ve found a
protocol used by the Children’s Hospital of Philadelphia Pathology Core.
http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf


Two things:  Iodine crystals are out of the question. Can I replace that
with Lugol’s iodine solution or should I just substitute Weigert’s for the
hematoxylin in this protocol?


Also, saffron vs tartrazine vs Orange G stain.  There is a real difference
in price. Does anybody have a personal preference in terms of quality or
aesthetics? Is paying the extra money for saffron really worth it?


Thanks!

Angela



-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037