[Histonet] Histonet Digest, Vol 153, Issue 24 davidsons fixative fish

[Steve McClain]

Something sounds fishy
Dr. Richmond can solve it.
http://www.ihcworld.com/_protocols/histology/davidson_fixative.htm

Steve A. McClain, MD

On Aug 30, 2016, at 13:22, "histonet-request at lists.utsouthwestern.edu<mailto:histonet-request at lists.utsouthwestern.edu>" <histonet-request at lists.utsouthwestern.edu<mailto:histonet-request at lists.utsouthwestern.edu>> wrote:

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Today's Topics:

  1. pediatric facilities only (Houston, Ronald)
  2. Eosin (Cameron, Elizabeth)


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Message: 1
Date: Tue, 30 Aug 2016 11:42:10 +0000
From: "Houston, Ronald" <Ronald.Houston at nationwidechildrens.org<mailto:Ronald.Houston at nationwidechildrens.org>>
To: "histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>"
   <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Subject: [Histonet] pediatric facilities only
Message-ID:
   <E5D0CD2352E46545A0C0EBE308CCCE537526BC22 at L1PERDWXMB02.childrensroot.net<mailto:E5D0CD2352E46545A0C0EBE308CCCE537526BC22 at l1perdwxmb02.childrensroot.net>>

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If you do not work in a pediatric facility please disregard

I need some help getting statistics for benchmarking for staffing evaluation

What is your annual volume of cases/blocks?
Continual flow processing?
# of IHC/Special stains
EM?
Shifts?
Days only?
Weekend coverage?
How many HTs do you have?
How many PAs?
Lab Assistants?
How many pathologists?
Do you perform any research?
What do you do regarding transcription? # of transcriptionists, outsource, voice recognition?

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
Laboratory Services
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston at nationwidechildrens.org<mailto:ronald.houston at nationwidechildrens.org><mailto:ronald.houston at nationwidechildrens.org>
www.NationwideChildrens.org<http://www.nationwidechildrens.org><http://www.nationwidechildrens.org/>

"Without continual growth and progress, such words as improvement, achievement, and success have no meaning."
~ Ben Franklin



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Message: 2
Date: Tue, 30 Aug 2016 14:25:24 +0000
From: "Cameron, Elizabeth" <ecameron1 at midcoasthealth.com<mailto:ecameron1 at midcoasthealth.com>>
To: "histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>"
   <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Subject: [Histonet] Eosin
Message-ID:
   <B5F1B1BBFD0AAF43A0A9F660F1B421E4AE6295 at mchex2010.mch.midcoasthealth.com<mailto:B5F1B1BBFD0AAF43A0A9F660F1B421E4AE6295 at mchex2010.mch.midcoasthealth.com>>

Content-Type: text/plain; charset="us-ascii"

Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense.  Our standard protocol works well for our own tissue, but the fish look much more washed out.  I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin.  I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573



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End of Histonet Digest, Vol 153, Issue 24
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