[Histonet] Histonet Digest, Vol 153, Issue 15

[Jim Anzaldi]

Vendor is soliciting sales item #1 

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Sent: Thursday, August 18, 2016 1:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 153, Issue 15

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Today's Topics:

   1. update (Corey Hubbard)
   2. Re: Gallocyanine (Bob Richmond)
   3. Re: Gallocyanine (Elizabeth Chlipala)


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Message: 1
Date: Wed, 17 Aug 2016 14:15:29 -0400
From: Corey Hubbard <corey at rankinbiomed.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] update
Message-ID:
	<CAKSgtkf81Hv=jwsnFJqmYqwOmJe515QZ1uBq3RdcQBs6V+fS3g at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Hi Eva,

I wanted to see how things are going for you. Please let me know if you come across any equipment needs. We specialize in histology and pathology equipment and service.

We are also always looking to purchase equipment - broken, used, collecting dust. etc.

Do you have a vendor you use currently for equipment?



*Corey Hubbard *
Sales Account Manager / Marketing Admin
248.215.5390 ext 0022

*RANKIN*
14515 Mackey Road
Holly, MI 48442
www.rankinbiomed.com
facebook.com/rankinbiomed
twitter.com/rankinbiomed.com

*John 8:12* Jesus spoke to them, saying "I am the light of the world; he who follows me will not walk in darkness, but will have the light of life.


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Message: 2
Date: Wed, 17 Aug 2016 19:58:38 -0400
From: Bob Richmond <rsrichmond at gmail.com>
To: "Histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Gallocyanine
Message-ID:
	<CAOKsRH6Mi0ff3xC3NqgVifbTrUPhfM0EaAOxRcE0okhEvPF=6Q at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Eva Permaul at Georgetown University asks:

>>We just had a request for Gallocyanine staining. Does anyone do this? 
>>Can
you share your protocol? Where do you buy your reagents?<<

Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance.
Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it?

Bob Richmond
Samurai Pathologist
Maryville TN


------------------------------

Message: 3
Date: Thu, 18 Aug 2016 08:03:45 -0600
From: Elizabeth Chlipala <liz at premierlab.com>
To: Bob Richmond <rsrichmond at gmail.com>,
	"'histonet at lists.utsouthwestern.edu'
	(histonet at lists.utsouthwestern.edu)"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Gallocyanine
Message-ID:
	<14E2C6176416974295479C64A11CB9AE02BED535B69A at SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

We have run this stain before, purchased the reagent from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, August 17, 2016 5:59 PM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Gallocyanine

Eva Permaul at Georgetown University asks:

>>We just had a request for Gallocyanine staining. Does anyone do this? 
>>Can
you share your protocol? Where do you buy your reagents?<<

Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance.
Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it?

Bob Richmond
Samurai Pathologist
Maryville TN
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End of Histonet Digest, Vol 153, Issue 15
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