[Histonet] Gallocyanine

[Elizabeth Chlipala]

We have run this stain before, purchased the reagent from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, August 17, 2016 5:59 PM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Gallocyanine

Eva Permaul at Georgetown University asks:

>>We just had a request for Gallocyanine staining. Does anyone do this? 
>>Can
you share your protocol? Where do you buy your reagents?<<

Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance.
Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it?

Bob Richmond
Samurai Pathologist
Maryville TN
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