[Histonet] Freezing biopsies

[Morken, Timothy]

Judi, what is the purpose of freezing it? For sectioning, biochemistry, histochemistry, or shipping it? It makes a difference. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-----Original Message-----
From: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, August 11, 2016 10:23 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Freezing biopsies

Hi everyone,
Does anyone have experience working with human frozen biopsies? I have a question concerning freezing the biopsies. I am just doing general background research on this subject at the moment and have not worked with frozen human biopsies.

If a biopsy was taken using a 14 gauge needle, in general, what is the length and diameter of a biopsy and what would the preferred method of freezing be (flash freezing in dry ice or freezing in liquid nitrogen)? What is the method for flash freezing in dry ice? What gauge of needles are most commonly used?  Are most biopsies taken using a 16-18 gauge needle?

Thanks for your help with this. I really appreciate it.
Best regards,
Judi Ford


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