[Histonet] Cryostat sectioning of frozen leaves

[Jo-Ann Bader, Ms.]

I am trying to cut frozen leaf sections at 6 and 8 microns.  All I am getting is bunched up leaf.  What is the trick/  I am cutting at -20 should be cutting at a lower temp and cutting thicker?


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270