[Histonet] Reference for erythrosin-eosin counterstain for H&E

Jennifer MacDonald JMacDonald at mtsac.edu
Sun Apr 3 21:57:41 CDT 2016


There is a reference to erythrosin in Lynch's Medical Laboratory 
Technology, but it is brief.  Culling is the author of the histology 
chapter, so perhaps he has something more in depth.



From:   "Tony Henwood (SCHN) via Histonet" 
<histonet at lists.utsouthwestern.edu>
To:     "histonet at lists.utsouthwestern.edu" 
<histonet at lists.utsouthwestern.edu>
Date:   04/03/2016 05:20 PM
Subject:        [Histonet] Reference for erythrosin-eosin counterstain for 
H&E



Hi all,

I am doing an H&E workshop next month and one of the Hx counterstains I 
will be including is Erythrosin-eosin (see below).
This counterstain is the preferred by some of our major Histopathology 
departments in Australia.

The issue I have is that I cannot find a reference for this variant of the 
H&E.

Anyone have any ideas?


Eosin Y (CI 45380)                             5g
Erythrosin B (CI 45430)                   5g
Sodium Hydrogen Carbonate     1.25g
Magnesium Sulphate                     10g
Distilled water                                   500ml
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principle Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


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