[Histonet] Hematoxylin Precipitate and filtering Gill formulations

[Gayle Callis]

Yes, I have used Gill 1, 2 and 3 even in the early days of buying these
formulations from a vendor, and always filtered them before using.  

 Old school habits never changed..............   

Gayle Callis 

-----Original Message-----
From: Manfre, Philip via Histonet [mailto:histonet at lists.utsouthwestern.edu]

Sent: Tuesday, September 22, 2015 12:26 PM
To: Elizabeth Chlipala <liz at premierlab.com>; Tim H <thigginsht at msn.com>
Cc: 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Hematoxylin Precipitate

Wow, I agree with Liz.  There should not routinely be "so much tissue
washing off".  There is a fundamental problem, if this is the case.

With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3?
These do not need filtering and do not produce a precipitate.

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre at merck.com