[Histonet] Hematoxylin Precipitate

Tue Sep 22 13:26:04 CDT 2015

Wow, I agree with Liz.  There should not routinely be "so much tissue washing off".  There is a fundamental problem, if this is the case.

With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3?  These do not need filtering and do not produce a precipitate.

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre at merck.com

-----Original Message-----
From: Elizabeth Chlipala via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, September 22, 2015 1:55 PM
To: Tim H; 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)
Subject: Re: [Histonet] Hematoxylin Precipitate

I think you may a have a serious problem if "so much tissue washes off"  if that is happening  you have problems with tissue adherence.  A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off.  That would mean that you did not provide to the pathologist what is represented in the block.  If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, September 22, 2015 11:25 AM
To: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Precipitate

You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says.  We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed.  So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides.

You can also try increasing your rinse times and see if that doesn't help as well.  

Thanks,

Tim
> 
> Message: 1
> Date: Mon, 21 Sep 2015 15:14:39 -0500
> From: "Sandra Cheasty" <cheastys at svm.vetmed.wisc.edu>
> To: "Histonet (histonet at lists.utsouthwestern.edu)"
> 	<histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Hematoxylin Precipitate
> Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello all,
> 
>                 Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive.
> 
>                 Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome.
> 
> Cheers,
> 
> Sandy
> 
>  
> 
> Sandra J. Cheasty, HT (ASCP)
> 
> Histology & Necropsy Supervisor
> 
> UW-Madison, School of Veterinary Medicine

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