[Histonet] Hematoxylin Precipitate

[Tim H]

You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says.  We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed.  So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides.
 
You can also try increasing your rinse times and see if that doesn't help as well.  
 
Thanks,
 
Tim
> 
> Message: 1
> Date: Mon, 21 Sep 2015 15:14:39 -0500
> From: "Sandra Cheasty" <cheastys at svm.vetmed.wisc.edu>
> To: "Histonet (histonet at lists.utsouthwestern.edu)"
> 	<histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Hematoxylin Precipitate
> Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello all,
> 
>                 Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive.
> 
>                 Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome.
> 
> Cheers,
> 
> Sandy
> 
>  
> 
> Sandra J. Cheasty, HT (ASCP)
> 
> Histology & Necropsy Supervisor
> 
> UW-Madison, School of Veterinary Medicine