[Histonet] axolotl lymphatics and Okada article

[Jason Palmer]

Hi all, 

A few weeks back I posted a question about identification of lymphatics in the axolotl (see below). John Kiernan suggested that enzyme histochem on paraffin sections might be possible, as per an article by Okada et al in 1994. I have been trying to hunt down this article but so far to no avail. Does anyone have a copy they may be able to scan and send me? It is in a procedings supplement, Lymphology journal: 

Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. 

Book title is: 

Progress in Lymphology XIV—Proceedings of the XIVth Congress in Washington 
DC, 1993, MH Witte, CL Witte (Eds.): Lymphology 27(Suppl): 1-893, 1994. 

Many thanks , 

Jason 

-- 

Jason Palmer 
Histology Laboratory Coordinator 
O'Brien Institute / St Vincent's Institute 
42 Fitzroy St, Fitzroy Victoria 3065 
Australia 
tel +61 3 9288 4045 
fax +61 3 9416 0926 
email: jpalmer at svi.edu.au 
----- Original Message -----
From: "John Kiernan" <jkiernan at uwo.ca> 
To: "Jason Palmer" <jpalmer at svi.edu.au>, histonet at lists.utsouthwestern.edu 
Sent: Wednesday, 29 July, 2015 2:06:29 AM 
Subject: Re: [Histonet] axolotl lymphatics 

Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. 
Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blood vessels. Here are a few references. 

Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for identification of lymphatic capillaries. Lymphology 24 :125-129. 
Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic capillary. Lymphology 27 , Suppl:106-107. 
Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. 
Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the gastric wall. Journal of Histochemistry and Cytochemistry 51 :331-338. 

Needless to say, none of these relate to amphibian tissues! 

John Kiernan 
Anatomy, UWO, London, Canada 
= = = 
On 26/07/15, Jason Palmer via Histonet <histonet at lists.utsouthwestern.edu> wrote: 



Hi all, 

I need to find an antibody that will label lymphatic endothelial cells in the axolotl. Does anybody have any experience or ideas? I have tried a couple of our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no cross-reactivity so far. I have no experience with staining of non-mammalian tissues - maybe an anti-frog Ab would cross react? Does anyone have experience with other amphibians? 

Thanks, 

Jason 



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