[Histonet] Fatty breast tissue

Patrick Laurie foreightl at gmail.com
Thu Oct 8 09:04:22 CDT 2015

The key is to make sure that you have enough formalin fixation (greater
than 6 hours for ASCO/CAP standards for biomarker standards).  That is a
bare minimum, and if the sections are thick, usually unacceptable cutting
quality will result.  It would be best if the pathologist could wait at
least 12 or more hours, it will result in better morphology.  If the
pathologist insists on processing the same day, with minimum fixation,
perhaps set up a protocol on your processor that includes a couple of hours
of fixation (with pressure, can help) to add on.  More time in the
dehydration steps and clearing steps will also help. Remember, though, when
you calculate the formalin fixation times, you need to add the time that it
was on the processor too.

Good luck

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869

On Thu, Oct 8, 2015 at 7:52 AM, Demarinis, Carolyn via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> What are the best choices for processing fatty breast tissue when the
> pathologist grosses the tissue the same day it is excised?
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