[Histonet] FW: GMS and Gram stains

[Morken, Timothy]

________________________________________
From: Histology [histo at pathlab.us]
Sent: Monday, October 05, 2015 5:18 AM
To: Morken, Timothy
Subject: RE: GMS and Gram stains

1.  Gram:  Sigma-Aldrich Accustain
2.  Gram:  Hard to see gram negative

1. GMS:  Grocott's Methenmmine Silver modified
2. GMS:  Switched from microwave to oven.  Too long (2 hours) and can't seem to get the fungi dark enough.

Thanks

-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu]
Sent: Friday, October 02, 2015 12:16 PM
To: Histology
Subject: RE: GMS and Gram stains

Mehndi,
These procedures are so common that without more information you are likely to get a lot of people sending you procedures that are identical to what you now use.

The questions we will ask are:
1) what procedures are using now?
 and
2) what is it about your current procedures  that is not acceptable?



Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: Histology via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, October 02, 2015 4:59 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] GMS and Gram stains

Hi All Netters,

I need to get a better GMS and Gram stain.  If anyone could share their protocols where they get the solutions that would be fantastic.

Thanks,

Mehndi Helgren

Dominion Pathology Labs
Norfolk, VA (AKA Hurricane Central)  :)


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