[Histonet] FW: GMS and Gram stains

Morken, Timothy Timothy.Morken at ucsf.edu
Mon Oct 5 08:55:27 CDT 2015

From: Histology [histo at pathlab.us]
Sent: Monday, October 05, 2015 5:18 AM
To: Morken, Timothy
Subject: RE: GMS and Gram stains

1.  Gram:  Sigma-Aldrich Accustain
2.  Gram:  Hard to see gram negative

1. GMS:  Grocott's Methenmmine Silver modified
2. GMS:  Switched from microwave to oven.  Too long (2 hours) and can't seem to get the fungi dark enough.


-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu]
Sent: Friday, October 02, 2015 12:16 PM
To: Histology
Subject: RE: GMS and Gram stains

These procedures are so common that without more information you are likely to get a lot of people sending you procedures that are identical to what you now use.

The questions we will ask are:
1) what procedures are using now?
2) what is it about your current procedures  that is not acceptable?

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-----Original Message-----
From: Histology via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, October 02, 2015 4:59 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] GMS and Gram stains

Hi All Netters,

I need to get a better GMS and Gram stain.  If anyone could share their protocols where they get the solutions that would be fantastic.


Mehndi Helgren

Dominion Pathology Labs
Norfolk, VA (AKA Hurricane Central)  :)

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